|Tissue 1 (Library 1)||Tissue 2 (Library 1)||Tissue 3 (Library 2)||Tissue 4 (Library 2)|
|Tissue 5 (Library 3)||Tissue 6 (Library 3)||Tissue 7 (Library 4)||Tissue 8 (Library 4)|
|Tissue 9 (Library 5)||Tissue 10 (Library 5)||Tissue 11 (Library 6)||Tissue 12 (Library 6)|
Next-generation sequencing was conducted on six different tissue samples at the Genomic Lab of the David H. Murdock Research Institute(http://dhmri.org/).
For the sequencing, 6 cDNA Libraries have been prepared from the following tissues of Uca minax (commonly known as the red-jointed fiddler crab):
These libraries were then combined together to generate one unified library pool for sequencing.
A 100 Bp paired end read sequencing run was conducted on each individual tissue using the Illumina HiSeq2000 instrument. This yielded 12 files each containing over 7 million sequences in fastq format, each occupying over 1.8G of storage. The Sequence data quality control is: